Cytochrome P4501A (CYP1A) in Killifish (Fundulus heteroclitus): Heritability of Altered Expression and Relationship to Survival in Contaminated Sediments
Identifieur interne : 000573 ( Main/Exploration ); précédent : 000572; suivant : 000574Cytochrome P4501A (CYP1A) in Killifish (Fundulus heteroclitus): Heritability of Altered Expression and Relationship to Survival in Contaminated Sediments
Auteurs : Joel N. Meyer ; Diane E. Nacci [États-Unis] ; Richard T. Di GiulioSource :
- Toxicological Sciences [ 1096-6080 ] ; 2002-07.
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Abstract
Previous research has shown that killifish (Fundulus heteroclitus) inhabiting a creosote-contaminated site on the Elizabeth River in Virginia exhibit little induction of cytochrome P4501A (CYP1A) protein expression and activity upon exposure to typical CYP1A-inducing chemicals. We characterized the CYP1A response of first, second, and third generation laboratory-raised offspring of feral Elizabeth River killifish to exposure to sediments from the contaminated site as well as the prototypical polycyclic aromatic hydrocarbon (PAH)-type CYP1A inducers β-naphthoflavone (BNF) and 3-methylcholanthrene (3-MC). The Elizabeth River offspring's responses were compared to those of offspring of killifish from two reference sites (King's Creek, Virginia, and Russell Creek, North Carolina). As with feral Elizabeth River killifish, the first generation embryos and larvae were refractory to CYP1A induction. However, the response observed in 3-year-old first generation adults, as well as with second and third generation fish, was much closer to that observed in reference-site fish. We suggest that the pattern of altered CYP1A response in Elizabeth River killifish, while persistent and heritable for one generation, is mostly nongenetically based. Additionally, we investigated the hypothesis that low CYP1A activity (measured as in ovo EROD activity) would correlate to increased survival in Elizabeth River sediment pore water; this hypothesis was not supported by our results.
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DOI: 10.1093/toxsci/68.1.69
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Meyer</name><affiliation><wicri:noCountry code="subField">27708–0328; and</wicri:noCountry></affiliation></author><author><name sortKey="Nacci, Diane E" sort="Nacci, Diane E" uniqKey="Nacci D" first="Diane E." last="Nacci">Diane E. Nacci</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Rhode Island</region></placeName><wicri:cityArea>United States Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Atlantic Ecology Division, Narragansett</wicri:cityArea></affiliation></author><author><name sortKey="Di Giulio, Richard T" sort="Di Giulio, Richard T" uniqKey="Di Giulio R" first="Richard T." last="Di Giulio">Richard T. Di Giulio</name><affiliation><wicri:noCountry code="subField">27708–0328; and</wicri:noCountry></affiliation></author></analytic><monogr></monogr><series><title level="j">Toxicological Sciences</title><title level="j" type="abbrev">Toxicol. Sci.</title><idno type="ISSN">1096-6080</idno><idno type="eISSN">1096-0929</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2002-07">2002-07</date><biblScope unit="volume">68</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="69">69</biblScope><biblScope unit="page" to="81">81</biblScope></imprint><idno type="ISSN">1096-6080</idno></series><idno type="istex">681675820501CED59B03649B6B32FA2A8D29558D</idno><idno type="DOI">10.1093/toxsci/68.1.69</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1096-6080</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Fundulus heteroclitus</term><term>acclimation</term><term>adaptation</term><term>creosote</term><term>cytochrome P4501A</term><term>killifish</term><term>mummichog</term><term>nongenetic inheritance</term><term>polycyclic aromatic hydrocarbons</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Previous research has shown that killifish (Fundulus heteroclitus) inhabiting a creosote-contaminated site on the Elizabeth River in Virginia exhibit little induction of cytochrome P4501A (CYP1A) protein expression and activity upon exposure to typical CYP1A-inducing chemicals. We characterized the CYP1A response of first, second, and third generation laboratory-raised offspring of feral Elizabeth River killifish to exposure to sediments from the contaminated site as well as the prototypical polycyclic aromatic hydrocarbon (PAH)-type CYP1A inducers β-naphthoflavone (BNF) and 3-methylcholanthrene (3-MC). The Elizabeth River offspring's responses were compared to those of offspring of killifish from two reference sites (King's Creek, Virginia, and Russell Creek, North Carolina). As with feral Elizabeth River killifish, the first generation embryos and larvae were refractory to CYP1A induction. However, the response observed in 3-year-old first generation adults, as well as with second and third generation fish, was much closer to that observed in reference-site fish. We suggest that the pattern of altered CYP1A response in Elizabeth River killifish, while persistent and heritable for one generation, is mostly nongenetically based. Additionally, we investigated the hypothesis that low CYP1A activity (measured as in ovo EROD activity) would correlate to increased survival in Elizabeth River sediment pore water; this hypothesis was not supported by our results.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Rhode Island</li></region></list><tree><noCountry><name sortKey="Di Giulio, Richard T" sort="Di Giulio, Richard T" uniqKey="Di Giulio R" first="Richard T." last="Di Giulio">Richard T. Di Giulio</name><name sortKey="Meyer, Joel N" sort="Meyer, Joel N" uniqKey="Meyer J" first="Joel N." last="Meyer">Joel N. Meyer</name></noCountry><country name="États-Unis"><region name="Rhode Island"><name sortKey="Nacci, Diane E" sort="Nacci, Diane E" uniqKey="Nacci D" first="Diane E." last="Nacci">Diane E. Nacci</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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